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a cyanoethyl (CE) protecting group on cytosine
which can cleave the silyl group without damaging the RNA backbone
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[1] Beaucage
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DMT-2'-F-dG(iBu)-CE Phosphoramidite 2'-Fluoro Phosphoramidites a cyanoethyl (CE) protecting groupDMT 2 F dG(iBu) CE Phosphoramidite is a 2' fluoro (2' F) phosphoramidite monomer used for solid phase oligonucleotide synthesis. It contains a 2' F modification on the sugar of deoxyguanosine (dG) with isobutyryl (iBu) base protection, a 5 dimethoxytrityl (DMT) group for 5' hydroxyl protection, and a cyanoethyl (CE) protecting group on phosphite. Synthesis of RNA oligonucleotides using 2' F phosphoramidite increases the nuclease resistance and binding
